Hi everyone Has anyone used monoclonal anti-calbindin from Sigma on cryos? What fixation is best? PFA? Thanks in advance! Inga -- Inga Hansson dept. neuroscience, div. neurobiology PO Box 587 Biomedical Centre Husargatan 3 S-751 23 Uppsala SWEDEN phone:+46-18-4714384 fax: +46-18-559017