[Histonet] Frozen cell pellet

[Favara, Cynthia (NIH/NIAID)]

I have done as you have, also spin down add a bit of histogel vortex let
cool then flick out and put in OCT. I like this as I seem to get a more even
distribution of cells.

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Susan Q Wells [mailto:susan.wells <@t> bms.com] 
Sent: Tuesday, November 30, 2004 11:22 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Frozen cell pellet

Does anyone have advise on how to freeze cells that have been spun down in a
micro centrifuge tube? In the past I've snap frozen the tube, loosened the
cells by flicking the tube and then embedded the pellet in OCT. The cells
look OK, but the cells in the middle don't look as good as the ones on the
perimeter.Any advise would be appreciated.
Thanks,
Sue Wells

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