[Histonet] Counterstain for Von Kossa

[Tony Henwood]

For Von Kossa, there are many counterstains you can use:
Nuclear Fast Red, Ethyl (was Methyl) Green (pH 4.2), even a light eosin.
Take your pick.

What other structures do you want to co-observe (Is that a word?)?

Regards

Tony Henwood JP, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: Fredrik Johansson [mailto:fredrik <@t> u.washington.edu] 
Sent: Tuesday, 30 November 2004 4:52 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Counterstain for Von Kossa


Hi,

 

I am going to stain 5 um paraffin sections of mouse arteries for 
calcium (Von Kossa) and need a counterstain. I'm new in this area and 
would appreciate if some one could tell me were to find a protocol for 
this. I have looked in the archive and found a protocol for bone 
sections (toluidine blue) can I use the same protocol for my mouse 
arteries?

 

Thanks

 

Fredrik Johansson

Department of Pathology

UW

Seattle


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


**********************************************************************
This email and any files transmitted with it are confidential and
intended solely for the use of the individual or entity to whom they
are addressed. If you are not the intended recipient, please 
delete it and notify the sender.

Views expressed in this message and any attachments are those 
of the individual sender, and are not necessarily the views of The
Children's Hospital at Westmead

This footnote also confirms that this email message has been 
virus scanned and although no computer viruses were detected,
the Childrens Hospital at Westmead accepts no liability for any 
consequential damage resulting from email containing computer 
viruses.
**********************************************************************