[Histonet] Water contamination

[Jose Luis Palazon Fernandez]

In my lab we normally use 95 % EtOH before absolute alcohols, instead of the 70 % you use, without any problem. Maybe your contaminating agent is 70 % EtOH, try changing this step with a 95 % one

José Luis


El dia 27/11/2004 19:32 usted envio el siguiente mensaje:
>Date: 27 de Noviembre de 2004 19:32:07
>From: Paula Pierce <contact <@t> excaliburpathology.com> 
>Subject: [Histonet] Water contamination 
>To: histonet <@t> lists.utsouthwestern.edu
>
> First, you SLICE meat. Histotechs SECTION tissue.
>  
> 70% EtOH is 30% water. You are probably getting too much water carryover into your 100%. Make sure the 100% EtOH is 100%. If the solutions are fresh, 10 to 20 dips each is enough time for dehydration before xylene and coverslipping. 
> 
> 
> Paula Pierce, HTL(ASCP)HT
> 
> Excalibur Pathology, Inc.
> 631 N. Broadway
> Moore, OK 73160
> 405-759-3953
> contact <@t> excaliburpathology.com
> www.excaliburpathology.com
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Universidad de Oriente-Isla Margarita-Venezuela
actualmente en: Instituto de Ciencias Marinas de Andalucia
Puerto Real, Cádiz, España.
email: jluis.palazon <@t> icman.csic.es