[Histonet] F4/80 on formalin fixed frozen spleen

[Patsy Ruegg]

I use this antibody on ffpe ms tissues using proteinase K digestion for 5
min. no HIER.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Andrew
Wong
Sent: Friday, November 26, 2004 6:55 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] F4/80 on formalin fixed frozen spleen


I've had good results with F4/80 staining on formaldehyde fixed
cryostat sections of mouse brain.
However, it did require microwave/citric acid antigen retrieval.
Trypsinisation pre-treatment also works, but I found it to be harsher
on the sections than microwaving.

Regards,
Andrew

--
Andrew Wong
Division of Neuroscience
University of Edinburgh
1 George Square
Edinburgh EH8 9JZ
Andrew.Wong <@t> ed.ac.uk
T: 0131 6511 875
>
> Message: 6
> Date: Wed, 24 Nov 2004 13:40:40 -0500
> From: Sharon Cooperman <scoop <@t> mail.nih.gov>
> Subject: [Histonet] F4/80 on formalin fixed frozen spleen
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <p0602040fbdca84affbd4@[156.40.160.33]>
> Content-Type: text/plain; charset="us-ascii" ; format="flowed"
>
> Does anyone know if F4/80 can work on formalin fixed frozen mouse
> spleen for IHC without antigen retrieval?
>
> Also, thanks to everyone for their suggestions re. mouse B-cell
> markers.
>
> Thanks,
> Sharon
> --
> Sharon Cooperman        	     <scoop <@t> mail.nih.gov>
> NIH, NICHD, CBMB                     301.435-8417
> Building 18T, room 101               301.402-0078 fax
> Bethesda, MD 20892
>
>
>
>


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