[Histonet] IHC on Sections Processed for EM?

[Geoff McAuliffe]

Hi Michael:

    As usual, it "depends on the antigen". There are a few antigens that 
survive glut and osmium, I know that GFAP can be made to work on some 
astrocytes if the osmium is removed. Other than that, I would think a 
literature search for the antigen in question is the best first step. 
Maybe someone has made it work? Of course, the Epon will have to be etched.

Geoff

Michele French wrote:

> Good Morning Histonet! A colleague of mine did some EM work and found 
> something interesting. Our pathologist wanted me to try to do some IHC 
> to further characterize what is present in the section. Our EM person 
> said it would never work. I did not think it was possible either, but 
> I thought I would ask anyway. Has anyone tried doing an immunostain on 
> plastic (Epon) sections from tissue fixed and processed for EM? I am 
> always up for a challenge, but I am really busy right now and don't 
> want to waste my time if there is really no hope! Thanks in advance,  
> Michele French
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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