[Histonet] Elution reagent for Double Staining

[Gayle Callis]

Talk to Chris van der Loos - he has done this and actually feels this does 
NOT work very well.  There are other ways to get excellent results with 
double IHC and not use elution.  He can be reached at "C.M. vander Loos" 
<c.m.vanderloos <@t> amc.uva.nl>.  There are problems with elution.  One is the 
antibody is bound so strongly to antigen, it will not elute.  Another 
is  damage to antigens with elution reagents.  Chris is a master at double 
staining and taught this in Toronto this past year - look for him to do 
this again in Fort Lauderdale, NSH symposium 2005 - he did discuss elution 
and how to get around it.

A good visit with him may help you with your double staining.

At 07:33 AM 11/23/2004, you wrote:
>Anyone out there willing to share what they use for elution reagent during 
>double immunostaining using peroxidase and phosphatase systems 
>together?  I have looked and gotten a variety of answers, I would like to 
>know what actually works for people.  Thanks in advance -
>
>
>Amy S.Porter, HT(ASCP)
>Michigan State University
>Department of Physiology
>Division of Human Pathology
>College of Human Medicine
>portera203 <@t> yahoo.com
>
>
>
>---------------------------------
>Do you Yahoo!?
>  Meet the all-new My Yahoo! ­ Try it today!
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367 (lab with voice mail)
406 994-4303 (FAX)