[Histonet] antibodies

[Jim Staruk]

We've also used the same antibody, same pre-treatment at a 1:50 dilution
with very nice results.

Jim

___________________
     Jim Staruk
Mass Histology Service
www.masshistology.com
 
 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Elizabeth Chlipala
Sent: Thursday, November 18, 2004 4:34 PM
To: 'Patsy Ruegg'; 'Histonet'
Subject: RE: [Histonet] antibodies

Patsy

F4/80 will stain mouse macrophages, both fixed (kupfer) and mobile
macrophages, I would suspect they would also stain the macrophage
dendritic cells.  The antibody from Serotec will work on formalin fixed
tissue with proteinase K digestion. There might be an antibody out there
that is more specific than this one, but I'm not aware of any.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, Colorado 80308
Office: (303) 735-5001
Fax: (303) 735-3540
liz <@t> premierlab.com
www.premierlab.com
 
Ship to Address:
Premier Laboratory
University of Colorado
MCDB, Room A3B40
Boulder, Colorado 80309

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Patsy
Ruegg
Sent: Thursday, November 18, 2004 12:12 PM
To: 'Histonet'
Subject: [Histonet] antibodies

I am looking for antibody recommendations that work on ffpe mouse
tissues for:
macrophages
dendritic cells
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