Fwd: Re: [Histonet] Envision.

[Ian Montgomery]

>
>Question:  what buffer are you using?   If you use PBS, then you will have 
>phosphate ions available for AP, TRIS buffered saline is preferred with AP 
>protocols.
Gayle,
         I'm using TBS as suggested by Dako. With and without Tween, but 
still the same result.



>At 08:52 AM 11/18/2004, you wrote:
>>         I wonder if anyone can offer any hints and tips for Dako 
>> Envision. Localisation is fine, HRP is clean but alkaline phosphatase, 
>> despite my efforts, is still giving me quite a lot of background. This 
>> manifests itself as a fine red ppt. over the specimen.
>>Ian.
>>
>>Dr. Ian Montgomery,
>>Histotechnology,
>>Graham Kerr Building,
>>Institute of Biomedical & Life Sciences,
>>University of Glasgow,
>>Glasgow,
>>G12 8QQ.
>>Tel: 0141 339 8855
>>Office: 4652
>>Lab: 6644.
>>Pager: 07625 702883
>>e-mail: ian.montgomery <@t> bio.gla.ac.uk
>>
>>_______________________________________________
>>Histonet mailing list
>>Histonet <@t> lists.utsouthwestern.edu
>>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>Gayle Callis
>MT,HT,HTL(ASCP)
>Research Histopathology Supervisor
>Veterinary Molecular Biology
>Montana State University - Bozeman
>PO Box 173610
>Bozeman MT 59717-3610
>406 994-6367 (lab with voice mail)
>406 994-4303 (FAX)
>

Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery <@t> bio.gla.ac.uk