[Histonet] Envision.

[Joanne Mauger]

Dr. Montgomery,
Have you tried adding levamisole to your chromogen? It blocks endogenous alk-phos in tissue.Dako sells it seperately from kit. I use 1 drop per ml. of chromogen, and it works quite well.
Jo

>>> "Ian Montgomery" <ian.montgomery <@t> bio.gla.ac.uk> 11/18/04 10:52AM >>>
         I wonder if anyone can offer any hints and tips for Dako Envision. 
Localisation is fine, HRP is clean but alkaline phosphatase, despite my 
efforts, is still giving me quite a lot of background. This manifests 
itself as a fine red ppt. over the specimen.
Ian.

Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery <@t> bio.gla.ac.uk 


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