[Histonet] re: HELP!!! - Autofluorescence in the mouse brain

[Mike King]

Pawel,
Sounds like you might be seeing lipofuschin granules, common 
autofluorescent intracellular structures in brain.  To reduce 
autofluroescence see
Baschong W, Suetterlin R, Laeng RH.  Control of autofluorescence of 
archival formaldehyde-fixed, paraffin-embedded tissue in confocal laser 
scanning microscopy (CLSM).  J Histochem Cytochem. 2001 Dec;49(12):1565-72.
They describe studies using ammonia-ethanol, borohydride, and
sudan Black B alone or in combination, with a variety of fixation and 
section prep methods compatible with immunolabeling.  You can find other 
literature regarding the application of these reagents to 
autofluorescence problems.  Expect some trial-and-error to figure out 
what works best for your particular specimens.