[Histonet] Agitation of cassettes

[Patsy Ruegg]

I too have always processed most of my tissue by hand (usually bone) and
swear by using a shaker for all steps, fixation, decal, etc.  For mass
processing using a tissue processor mine has a stir bar at the bottom of
the tank and I do choose vacuum when ever possible.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Molinari, Betsy
Sent: Friday, November 12, 2004 6:08 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Agitation of cassettes


Hi Fran,
Happy Friday to you! We do put our cassettes on a shaker. I work in a
research lab and have the luxury of time (well, most of the time I do)
to do so. I usually just keep the cassettes on overnight. I always put
my decals on a rotator or shaker it cuts my decal time by quite a bit. I
do not have a publication, but I am someone on this site will! Betsy
Molinari HT(ASCP) Texas Heart Institute Cardiovascular Pathology
Houston,TX 77030 832-355-6524


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Fran
Lemons
Sent: Thursday, November 11, 2004 2:20 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Agitation of cassettes)

Hello all, it's almost Friday!!

Has anyone out there ever heard of placing the basket of cassettes in
formalin on an agitator to stir the formalin as a way to expedite
fixation?  If so, can you direct me to something published on the
subject? Also, has anyone heard of the same thing for bunions in decal
solution? Thanks ahead of time, Fran Walker


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