[Histonet] CD61 problems

[Drew Sally A.]

I was wondering if anyone else has had any problems with getting CD61(Y2/51) to work on Z-5 fixed
bone marrow biopsies.  Only 1 out of 8 cases I have tried gave positive staining in the megakaryocytes.
The histology lab uses RDO for decal'ing...  I believe the process the specimens go through involves the 
bone marrow lab putting the biopsy in Z-5 for...before transferring them to surgical pathology.  
The biopsies are then decal'ed for 30 min., rinsed and then put into neutral buffered formalin.

Sally Ann Drew, MT(ASCP)
IHC/ISH Laboratory
University of Wisconsin Hosp. & Clinics
Madison, WI 53792
(608)265-6596