[Histonet] Oil Red O question

[Jackie M O'Connor]

I have never done Oil Red O on anything but frozen sections, since the 
alcohol steps in processing removes the fat.  But, it's been awhile - have 
I missed something new?

Jackie




"Morken, Tim - Labvision" <tpmorken <@t> labvision.com>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
11/10/2004 11:37 AM

 
        To:     "'Andrea Grantham'" <algranth <@t> u.arizona.edu>
        cc:     histonet <@t> lists.utsouthwestern.edu
        Subject:        RE: [Histonet] Oil Red O question


Andrea, in short, yes, fat can wash away in this stain. If you want to
quantitate, I suggest fixing in formalin and then in osmium BEFORE
processing to paraffin ( as you would for EM). Then the fat is immoblized
and appears dark grey or greenish on the paraffin H&E section. The
morphology is good and quanititation is much easier and more reliable.

Tim Morken
Lab Vision - Neomarkers
www.labvision.com

Free webhosting for US State Histotechnology Societies:
http://www.labvisioncorp.com/demowebsite/index.cfm

-----Original Message-----
From: Andrea Grantham [mailto:algranth <@t> u.arizona.edu] 
Sent: Wednesday, November 10, 2004 9:25 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Oil Red O question


This may be a crazy question. I'm doing mouse livers for a lab here and 
they requested Oil Red O. Did the stain - looked great. They are asking if 

it was possible that some of the fat washed away during the washing steps. 

The experiment requires that they quantitate the fat found in these 
sections. Is this a possibility? Is there a better way to quantitate fat?
For the record - my washing steps are done under a gentle stream of tap 
water running into a corner of the stain dish or coplin jar - not a full 
force gush of water from the faucet directly on the slides. Thanks. Andi
Grantham
.....................................................................
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: Sr. Research Specialist       University of Arizona               :
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