[Histonet] antigen for B-cells in bone marrow

[Bryan Hewlett]

John,

You are correct!
CD79a exhibits a wider range of B cell staining than CD20, labelling both
earlier and later forms.

Regards,

Bryan

----- Original Message -----
From: "JOHN PHILLIPS" <JOHN.PHILLIPS <@t> new-tr.wales.nhs.uk>
To: "Mildred Fail" <failm <@t> musc.edu>; <scoop <@t> mail.nih.gov>;
<histonet <@t> pathology.swmed.edu>
Sent: Wednesday, November 10, 2004 4:47 AM
Subject: RE: [Histonet] antigen for B-cells in bone marrow


I note someone has advocated CD20 staining for Bcell in bone marrow:

I do not profess to know much about these matters but from my limited
experience in performing immuno work on trephine biopsies i think that early
Bcell forms may be missed if CD20, is investigated on its own. CD79 antibody
will label early B cell precursor cells and so should be performed in
conjunction with or perhaps instead of CD20. That is where my knowedge and
understanding fails me.

I have chosen to contact the "Histonetters" who i am sure will sort this out
and as a consequence i will learn from sticking my oar in. That's what this
is all about, ain't it.

John, Wrexham, Wales.

-----Original Message-----
From: Mildred Fail [mailto:failm <@t> musc.edu]
Sent: 09 November 2004 21:47
To: scoop <@t> mail.nih.gov; histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] antigen for B-cells in bone marrow


CD20- clone L-26,DAKO, 1:2000  HIER with Citrate Buffer. We do it on BMs
almost every day, beautiful every time
Rena Fail


>>> Sharon Cooperman <scoop <@t> mail.nih.gov> 11/05/04 06:42PM >>>
Dear Histonetters,

Does anyone know of an antibody that works well on B-cells in bone
marrow?  I'm actually trying to use it on a western blot, but I think
many Abs that work on IHC work on western.  I've tried CD11a, CD11b,
and Pax-5, all of which are supposed to work on western but none did
(maybe I need to load more protein).  Any suggestions would be
greatly appreciated.

Thanks,
Sharon
--
Sharon Cooperman             <scoop <@t> mail.nih.gov>
NIH, NICHD, CBMB                     301.435-8417
Building 18T, room 101               301.402-0078 fax
Bethesda, MD 20892

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