[Histonet] IHC on cell cultures

[Jackie M O'Connor]

Can anyone help me with a protocol for preparing cell culture samples for 
IHC?  I'm particularly interested in looking at CD45.  I'm not quite sure 
of the best way to prepare the cells.  I can do cytospins - and I'll bet I 
have to fix the cells prior to cytospinning them.  If anyone has a 
protocol they'd like to share for preparing good slides from live cells, 
I'd appreciate it.  I'd like to keep the option of using cytospins or 
smears as well as making a cell block.  I'm experienced with making these 
preparations from body fluids, but not cell cultures.  Thanks.

Jackie O'.