[Histonet] HMB50 and D2-40

[B T]

thank you.......we have tried that protocol as well as a host of others....I 
have just retrieved offline and stained by hand as well....all to no avail!! 
I think the  antibody may be a dud.....since we have tried just about 
everything apart from standing on one foot and singing Jingle Bells while 
the slides "stain". Thanks for the info....it is very helpful.

The D2-40 is supposed to be a vascular marker....this is what the ordering 
pathologist intends to use it for.

Thanks again for your help......this supports my feeling that we got a bad 
batch of antibody.

Barb

>From: "Bill Sinai" <bills <@t> icpmr.wsahs.nsw.gov.au>
>To: "'B T'" <histobabs <@t> hotmail.com>,"histonet (E-mail)" 
><histonet <@t> lists.utsouthwestern.edu>
>Subject: RE: [Histonet] HMB50 and D2-40
>Date: Fri, 5 Nov 2004 15:44:36 +1100
>
>
>bARB,
>
>I have spoken to the lady who does ourIHC on the Benchmark and she uses the
>HMB45 + 50 antibody from Neomarkers.  She uses a mild CC1, the antibody
>diluted 1:50 and a 32min incubation time.
>
>Hope this helps.
>
>What is the other D2-40 antibody?
>
>Bill Sinai
>Laboratory Manager
>Tissue Pathology, ICPMR
>Westmead NSW 2145
>Australia
>Ph 02 9845 7774
>
>
>-----Original Message-----
>From: histonet-bounces <@t> lists.utsouthwestern.edu
>[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of B T
>Sent: Friday, 05 November 2004 8:26 AM
>To: histonet <@t> lists.utsouthwestern.edu
>Subject: [Histonet] HMB50 and D2-40
>
>
>Hi all,
>
>Is anyone out there doing melanoma clone HMB50 on the Ventana Benchmark? If
>so, what protocol are you using? I am having no success working up this
>antibody and at this point i'm not sure if there might be a problem eith 
>the
>antibody itself.
>
>Also, is anyone using D2-40 on the Benchmark......I am getting staining but
>would like to punch it up a notch without having the tissue fall off the
>slide.
>
>Help please!
>
>Barb
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet <@t> lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>__________________________________________________________________
>
>This electronic message and any attachments may be confidential.  If you
>are not the intended recipient of this message would you please delete the
>message and any attachments and advise the sender. Western Sydney
>Area Health Services (WSAHS) uses virus scanning software but excludes
>any liability for viruses contained in any email or attachment.
>
>This email may contain privileged and confidential information intended
>only for the use of the addressees named above. If you are not the
>intended recipient of this email, you are hereby notified that any use,
>dissemination, distribution, or reproduction of this email is prohibited. 
>If
>you have received this email in error, please notify WSAHS
>immediately.
>
>Any views expressed in this email are those of the individual sender
>except where the sender expressly and with authority states them
>to be the views of WSAHS.