[Histonet] Zinc.

[Jackie M O'Connor]

I've been forced to use a zinc only fixative (Streck Tissue Fixative) for 
a couple of years - BD (Pharmingen) recommends the use of this for many of 
it's antibodies - i.e. Caspase-3 and  CD31 - works great, but the 
morphology stinks.  I'm in the process of 'crossing over' to find 
antibodies that will work on FFPE as well on my xenografts. 
Jackie O'




Ian Montgomery <ian.montgomery <@t> bio.gla.ac.uk>
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
11/05/2004 08:54 AM

 
        To:     histonet <@t> lists.utsouthwestern.edu
        cc: 
        Subject:        [Histonet] Zinc.


         When choosing an antibody the companies usually give the 
preferred 
method of preparation, frozen, formalin, zinc etc. When they say zinc, do 
they mean a zinc only fixative or a zinc formalin mixture?
Ian.

Dr. Ian Montgomery,
Histotechnology,
Graham Kerr Building,
Institute of Biomedical & Life Sciences,
University of Glasgow,
Glasgow,
G12 8QQ.
Tel: 0141 339 8855
Office: 4652
Lab: 6644.
Pager: 07625 702883
e-mail: ian.montgomery <@t> bio.gla.ac.uk 


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