[Histonet] a much easier chemistry question

Geoff McAuliffe mcauliff <@t> umdnj.edu
Thu Nov 4 19:22:27 CST 2004


Soap and water?

Geoff

Jack England wrote:

> Aloha all,
>
> Here's a much more fun chemistry question for anyone that wants to 
> bite: what is the best method to remove trypan/evans blue from cotton 
> fabric?  One of my colleagues got some on her pants the other day and 
> asked what the best way to get it out was.  I did not know, so I put 
> some on cotton gauze, and tried:
>
> --acid alcohol...faded the stain a little, but didn't get rid of it
> --DI water...no change
> --absolute ethanol...no change
> --xylene...no change
> --ethanol/non-ionic detergent/DI water rinse...no change
> --citrus oil clearant...no change
>
> Given that her fabric is tan (and thus not white, and thus likely not 
> bleach-able), can anyone out there in histo-land suggest a way of 
> de-staining trypan-blue-stained fabric? I've been wondering about this 
> for a while now and figured I'd pass it around.
>
> --Many thanks and aloha to all,
> Jack England
> Tissue Genesis, Inc.
> http://www.tissuegenesis.com
>
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-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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