[Histonet] BMP7

[Patsy Ruegg]

Debra,
I have used Santa Cruz goat BMP-7 (L-19) sc-9305 on a lot of rat and
human samples but have not tried it on ms.  SC lists it as working for
rat and h, since it is a goat poly it would be easy to try it on your
ms, ask SC if anyone has reported it as working on ms although I don't
usually find SC being very helpful for anything not listed being tried.
For rats I use it at 1:200 for 1 hr. using LSAB+ detection (I wish
someone would come up with a labeled polymer that works with goat
antibodies) and pepsin digestion (premade from Phoenix) at 37dc for two
changes 5 min. ea.
Patsy

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Grant,
Debra
Sent: Wednesday, November 03, 2004 10:47 AM
To: histonet <@t> pathology.swmed.edu
Subject: [Histonet] BMP7



Has anyone used BMP7 antibody on mouse? If so, could you please send me
all the information, company, catalog #, protocol etc.

Thanks in advance!

Debby Grant
Research Technician II
Histology Core Facility
Stowers Institute for Medical Research
1000 E. 50th Street
Kansas City, MO 64110
drg <@t> stowers-institute.org
 

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