[Histonet] CD41, GPV, GP1bBeta
Emerson, Rachael
Rachael_Emerson <@t> URMC.Rochester.edu
Wed Nov 3 13:00:53 CST 2004
Hi. Does anyone have any experience working with CD41, GPV, or GP1bBeta
antibodies?
I could really use some help.
Thanks a lot!!
Rachael Emerson
Rachael L. Emerson
Center for Human Genetics and Molecular Pediatric Diseases
University of Rochester Medical Center
575 Elmwood Avenue MRBX 1.11301
Rochester, NY 14642
Tel (585) 275-5073
Fax (585) 276-0232
> ----------
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> Sent: Wednesday, November 3, 2004 1:03 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 12, Issue 4
>
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> Today's Topics:
>
> 1. Alcian Blue and steamer containers (Paula Pierce)
> 2. Re: Sta-On (Jennifer Sipes)
> 3. Epidermal growth factor (Ford, Rhonda)
> 4. Job posting - Dallas (Patterson, Pat)
> 5. RE: thanks and aquamount (Patsy Ruegg)
> 6. RE: AFB Stain (Joe Nocito)
> 7. Re: History of microtechnique (John Kiernan)
> 8. Re: GMA sectioning (Caroline Stott)
> 9. Re: sample preparation/analysis to observe gas bubbles
> (Wayne Kreider)
> 10. Re: Polyoma Virus (tdobersztyn <@t> chmca.org)
> 11. Re: AFB Stain (lpwenk <@t> sbcglobal.net)
> 12. Merkel cell staining (Marshall)
> 13. LR White vs LR Gold (Peter Rippstein)
> 14. RE: Modified Gomori's Trichrome (Sharon Allen)
> 15. Envision System with Vector Red Background (Amy Kozer)
> 16. Re: Merkel cell staining (Richard Cartun)
> 17. B & D steamer (Rita Angel)
> 18. Polyoma BK virus controls (Histology SLU)
> 19. BMP7 (Grant, Debra)
> 20. Thanks for the Teasing (Ant S.)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Tue, 2 Nov 2004 10:06:53 -0800 (PST)
> From: Paula Pierce <contact <@t> excaliburpathology.com>
> Subject: [Histonet] Alcian Blue and steamer containers
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20041102180653.38389.qmail <@t> web50308.mail.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Hello Andrew,
>
> yes I have already voted today :)
>
> You can buy a smaller amount of alcian blue at
> http://www.anatechltdusa.com/Catalog/catalogspecialstains.html
>
> I use plastic coplin jars in my B&D steamer for small quantities of slides
> and the larger tissue-tek when I have more.
>
>
> Paula Pierce, HTL(ASCP)HT
>
> Excalibur Pathology, Inc.
> 631 N. Broadway
> Moore, OK 73160
> 405-759-3953
> contact <@t> excaliburpathology.com
> www.excaliburpathology.com
>
> ------------------------------
>
> Message: 2
> Date: Tue, 2 Nov 2004 10:10:48 -0800 (PST)
> From: Jennifer Sipes <jengirl1014 <@t> yahoo.com>
> Subject: [Histonet] Re: Sta-On
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20041102181048.98641.qmail <@t> web60604.mail.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> I purchased it through a company called Surgi-Path. They're the ones that
> make it.
>
>
> Jennifer K. Sipes, RALAT
> Sr. Laboratory Technician
> Johns Hopkins University
> Ross 929
> 720 Rutland Avenue
> Baltimore, MD 21205
> phone: 410-614-0131
> cell: 443-413-0853
> e-mail: jengirl1014 <@t> yahoo.com
>
>
>
>
>
>
>
>
>
>
>
> ---------------------------------
> Do you Yahoo!?
> Check out the new Yahoo! Front Page. www.yahoo.com/a
>
> ------------------------------
>
> Message: 3
> Date: Tue, 2 Nov 2004 13:11:38 -0500
> From: "Ford, Rhonda" <RFORD <@t> HCMHCARES.ORG>
> Subject: [Histonet] Epidermal growth factor
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID:
> <684DF8CDE1FB6A428499DA65D753D12201F61789 <@t> JUPITER.HCMH.ORG>
> Content-Type: text/plain; charset="us-ascii"
>
> Does anyone know any institution(s) who perform this testing?
>
>
> ------------------------------
>
> Message: 4
> Date: Tue, 2 Nov 2004 12:26:35 -0600
> From: "Patterson, Pat" <PatPatterson <@t> mhd.com>
> Subject: [Histonet] Job posting - Dallas
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <293C7C19EFF7D611AE1A0002A53F81140CB43B0D <@t> omega.mhd.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
>
> Named one of the "Best Places to work 2004" by the Dallas Business
> Journal.
>
> Our dedicated team is very outspoken in their appreciation of the
> excellent
> clinical environment we foster and the ongoing commitment Methodist has
> made
> to patient satisfaction. Our dual focus on out patients and our employees
> is working - 2002 Methodist Health System earned the Gold Seal of
> Approval
> form the Joint Commission on Accreditation of Healthcare Organizations and
> more than 28 percent of our new hires come from employee referral! If
> excellent patient care is one of your priorities for career satisfaction,
> then you belong at Methodist.
>
>
>
> Join our Histology team at Methodist Dallas Medical Center. We're seeking
> a
> full-time Histology Technician on the day shift in our busy Anatomic
> Pathology Section. Job responsibilities include processing, embedding,
> sectioning, routine and special staining, assisting in gross room, frozen
> sectioning and computer entry into the MediTech lab information system.
> Experience with microwave technology would be an added plus. Requires
> HT/HTL
> ASCP registry or eligible. Minimum 2-year experience desired.
>
> Contact: Pat Patterson, Manager
> Phone: (214) 947-3538
> Fax: (214) 947-3524
> email: PatPatterson <@t> mhd.com
>
>
> ***********************************************************************
>
> This electronic transmission contains information from Methodist Health
> System and should be considered confidential and privileged. The
> information contained in the above messages is intended only for the
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> the intended recipient, be aware that any disclosure, copying,
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> this transmission in error, please notify the sender immediately by
> return e-mail. Methodist Health System, its subsidiaries and
> affiliates hereby claim all applicable privileges related to the
> transmission of this communication.
>
>
>
> ------------------------------
>
> Message: 5
> Date: Tue, 2 Nov 2004 11:33:43 -0700
> From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
> Subject: RE: [Histonet] thanks and aquamount
> To: "'Sharon Cooperman'" <scoop <@t> mail.nih.gov>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <001501c4c10a$7b4e4fa0$83020a0a <@t> IHCTech>
> Content-Type: text/plain; charset="us-ascii"
>
> Sharon,
> Aquamount will not harden, you should seal with clear nail polish to
> keep it permanently.
> Patsy
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sharon
> Cooperman
> Sent: Thursday, October 28, 2004 12:16 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] thanks and aquamount
>
>
> Dear Histonetters,
>
> Thanks to everyone for the great advice on bone decal and oil red o
> stain. I have one last question related to the oil red o stain - I
> just bought a bottle of aquamount to use when mounting the oil red o
> stained slides, but it didn't come with instructions (on the bottle
> it refers to instructions). Are there any special instructions for
> aquamount? Do I need to seal the slides with nail polish or does
> aquamount harden? Or, does anyone know how I can contact Lerner to
> get a copy of the instructions (I bought it from Fisher - Lerner
> doesn't answer the phone number I have for them).
>
> Thanks,
> Sharon
> --
> Sharon Cooperman <scoop <@t> mail.nih.gov>
> NIH, NICHD, CBMB 301.435-8417
> Building 18T, room 101 301.402-0078 fax
> Bethesda, MD 20892
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 6
> Date: Tue, 2 Nov 2004 12:58:15 -0600
> From: "Joe Nocito" <JNocito <@t> Pathreflab.com>
> Subject: RE: [Histonet] AFB Stain
> To: "Etheridge, Sandra AGF:EX" <Sandra.Etheridge <@t> gems8.gov.bc.ca>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <JFEMICGBHEGPLAMIJPJPIEDHCHAA.JNocito <@t> Pathreflab.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Sandra,
> attached is our procedure for Truant's AFB. We purchase the staining kit
> from Infolab.
>
>
>
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
> Etheridge, Sandra AGF:EX
> Sent: Tuesday, November 02, 2004 11:57 AM
> To: (histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] AFB Stain
>
>
> Hello, all,
>
> Does anyone have a current method for acid fast bacilli using
> auramine-rhodamine? Is this a fluorescent stain?
>
> Thanks in advance.
>
> Sandra Etheridge
>
> BC Ministry of Agriculture, Food & Fisheries
> Animal Health Center, Histology
> Abbotsford, BC
> Canada
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> Message: 7
> Date: Tue, 02 Nov 2004 14:19:49 -0500
> From: John Kiernan <jkiernan <@t> uwo.ca>
> Subject: Re: [Histonet] History of microtechnique
> To: MTitford <@t> aol.com
> Cc: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4187DDD5.6F8C39E1 <@t> uwo.ca>
> Content-Type: text/plain; charset=us-ascii
>
> There is also a chapter on the History of Staining
> by F. Kasten in the 10th edition of "Conn's
> Biological Stains." This book (2002) is
> still in print.
> -------------------------------
> John A. Kiernan
> Department of Anatomy and Cell Biology
> The University of Western Ontario
> London, Canada N6A 5C1
> _______________________________
> MTitford <@t> aol.com wrote:
> >
> > David Muskett asks about where to find information about the history of
> microtechnique.
> > A good place to start is with "A history of microtechnique" by Brian
> Bracegirdle. The second edition is still available here in the States from
> Science Heritage Limited, Lincolnwood, IL
> > a second good book is "History of staining" by George Clark and
> Frederick Kasten. 3ed edition, Williams & Wilkins, Baltimore & London. The
> second book is probably out of print but available on interlibrary loan.
> > There are many other sources but you can get information overload in
> this area!
> >
> > Mike Titford
> > USA Pathology
> > Mobile AL
>
>
>
> ------------------------------
>
> Message: 8
> Date: Wed, 03 Nov 2004 09:49:23 +1300
> From: Caroline Stott <caroline.stott <@t> anatomy.otago.ac.nz>
> Subject: [Histonet] Re: GMA sectioning
> To: Histonet <@t> lists.utsouthwestern.edu
> Message-ID: <5.2.1.1.0.20041103094227.02076a10 <@t> anatomy.otago.ac.nz>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Hi Ben,
> We have quite regularly cut GMA at 10, 20, 30 and 40 microns. We use a
> cotton bud to apply a little water to the face of the block, just gently
> rubbing it in. Also place some water on the glass knife as the section
> will come off easier. Then take a section and push it to the bottom of
> the
> water bowl/dish so it will flatten out. Then slide the sections onto the
> slide. Heres the tricky part.... We put a piece of filter paper on the
> bench then the slide on top, then another piece of wet filter paper on top
>
> of the slide (and sections) and use a roller (like a paint roller)
> applying a little pressure while rolling around 20-30 times over the slide
>
> and finally place on a hotplate (not flat) that is around 60 degrees
> C. Haven't had a problem. :)
> Hope that makes sense.
>
> Caroline
>
> Caroline Stott
>
> Histology Service Unit
> University of Otago
> PO Box 913
> Dunedin, New Zealand
> Ph (03) 479 7152
> Fax (03) 479 7136
>
> ------------------------------
>
> Message: 9
> Date: Tue, 02 Nov 2004 12:54:20 -0800
> From: Wayne Kreider <wkreider <@t> u.washington.edu>
> Subject: Re: [Histonet] sample preparation/analysis to observe gas
> bubbles
> To: Philip Oshel <peoshel <@t> wisc.edu>
> Cc: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4187F3FC.3060400 <@t> u.washington.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> Phil,
> Thanks again... all very helpful. I think we can now plan how we'll
> have the best chance at 'catching' transient bubbles. I'm not sure
> we'll have our techniques together for sampling and freezing the tissue
> in the very near term (this week), but I am hopeful we can do/try this.
> By the way, you mentioned that frozen samples are not useful for EM...
> how so? Perhaps due to subsequent fixing procedures? I'm relatively
> unfamiliar with EM although TEM had been suggested for this work.
> Wayne
>
> Philip Oshel wrote:
>
> > Wayne,
> >
> > You do have a problem ...
> > 2 X 2 X 2 mm is too big for any proper freezing method. Even
> > high-pressure freezing, which can in ideal conditions give the
> > greatest depth of good freezing, only does at best 500 microns. The
> > other methods do 200 microns or less. Plunging into LN2-cooled
> > cryogens will do a few 10s of microns, no more. They're fine for light
> > microscopy (assuming migration of molecules from holed membranes or
> > desiccation isn't an issue), but not EM.
> > The major source of freezing damage isn't really mechanical
> > ice-crystal growth and damage, but dehydration of cells from crystal
> > growth.
> > Plunging is literally dropping the sample into the cryogen, but not
> > really. By then I mean you *rapidly* plunge it in, not just let it
> > fall. By hand, as fast as possible. This is important in order to get
> > through the layer of cold nitrogen above the cryogen (whatever it is).
> > Some commercial plungers are designed to drop the sample into the
> > cryogen from enough height to pass through the cold gas layer quickly.
> > These are used for electrophysiology -- stimulate the sample then
> > drop, or stimulate during the drop, and therefore the sample is frozen
> > X msec after stimulation.
> > I can see doing this with ultrasonication, depending on the size, etc.
> > of the sonciation apparatus.
> > Cryoprotectants. Um. I don't think you can use these. The tissue has
> > to soak in the cryoprotectant -- often moving up a gradient -- in
> > order to get the cryoprotectant completely infiltrated into the
> > sample. This takes an hour or more -- maybe several hours? You'd
> > either have to assume nothing happens with the bubbles in the tissue
> > while sitting around infiltrating with cryoprotectant, or you'd have
> > to get the cryoprotectant into the tissue first, then sonicate and
> > pretend the cryoprotectant doesn't change the tissue properties and
> > therefore resulting bubbles.
> > I wouldn't believe either of those choices.
> > I think you'll need to do the experiment, then freeze, and use pieces
> > of tissue small enough to freeze properly. But! It's not entirely bad
> > -- only one dimension has to be =<200 microns -- 100 microns, really.
> > The other dimensions could be 2 mm.
> > So: 2 mm X 2 mm X 100 microns (even thinner would be better) should
> > work. Just hold the tissue by a corner, so that the cryogen has
> > maximal access to both faces of the thin side. And move the tissue
> > once in the slush LN2, don't hold it in one place -- keep fresh
> > cryogen contacting the sample.
> > Let's see -- oh. Insulate the container (and vacuum desiccator) as
> > best as possible, and work quickly, since you'll only have a few
> > minutes to freeze before the slush LN2 warms up to regular
> > almost-boiling LN2.
> > (I think Bal-Tec makes an apparatus for making slush LN2, and maybe
> > freezing with it, but you don't really need it, it just maybe makes
> > things easier.)
> >
> > Phil
> >
> > Phil,
> > Thanks for the input. Unfortunately, I haven't had much luck looking
> > in the archive.
> > The treatment is actually acoustic (high intensity ultrasound), so no
> > electrodes are involved. Our treatment volume that we'd ideally like
> > to freeze is about 2mm x 2mm x 5mm. However, from the little I've
> > read about plunging, a guideline for the maximum sample thickness is
> > 0.2mm. Using a slush as you describe would presumably help some, but
> > I suspect 2mm is still too thick.
> > If we are able to obtain a suitably thin sample, is plunging literally
> > just dropping the raw tissue into the cryogen? Or are there some
> > additional steps that should be taken in handling the sample?
> > Moreover, if we do have a larger sample, reference books suggest that
> > it's necessary to use a cryo-protectant. Is a cryo-protectant likely
> > to distort the tissue, in particular any bubble-type structures?
> > Also, what time delays are typically associated with using a
> > protectant? Any suggestions about which cryo-protectant might be best
> > suited for this application? Thanks again... I'd appreciate any
> > thoughts you might have.
> > Wayne
> >
> > Philip Oshel wrote:
> >
> > Cryofixation is the only way I know to preserve this kind of
> > structure. There have been discussions about this in the past, so you
> > might want to poke around in the Histonet archives.
> > What kind of treatment? Is it something that you stimulate with say an
> > electrode?
> > If so, there are plunge freezers designed to allow you to stimulate
> > the sample while it is falling into the liquid nitrogen.
> > Rapidity of freezing is essential to insure vitrification of the
> > water, instead of crystal formation. Although some folks maintain that
> > the water doesn't vitrify, but instead forms micro-crystallites small
> > enough to have no effect on structure. Others state that evanescent
> > microspherules are formed, sort of neither fish nor fowl -- not
> > crystalline and not glass. Either way, the frozen samples have to be
> > maintained below the recrystallization temperature (around -80deg C or
> > so) until after dehydraton or embedding to prevent crystal growth and
> > negating all the rapid freezing goodness.
> > But, the 4 basic methods are high-pressure freezing, propane-jet
> > freezing, slam-freezing, and plunging into cryogen. The first three
> > will likely distort the air bubbles and tissue around them, however.
> > Plunging works well, as long as the plunge is made very rapidly, and
> > no time is spent hanging about in the cold -- well below freezing --
> > nitrogen atmosphere above the cryogen.
> > Many people plunge into some organic fluid like ethane which is held
> > in a container in liquid nitrogen. This works, but it has a few
> > issues: the cryogen is usually warmer than the LN2, so the freezing is
> > not as rapid as it needs to be, and such organic cryogens are
> > flammable or explosive when they warm up. Especially since, being near
> > LN2 temperatures, they're enriching themselves in oxygen from the air
> > (liquid air is warmer than liquid nitrogen). They can be handled
> > safely, but this does require some thought.
> > A better way is to plunge into slush nitrogen -- LN2 near the freezing
> > point, instead of near the boiling point. This is about 14 deg C
> > colder than LN2, so freezes samples faster, and so gives a greater
> > depth of good freezing. It's also nitrogen, so there's no flammable
> > gas to deal with (nor bureaucrats upset about flammable gases in your
> > lab). It's easy to produce: just put a beaker full in a small to
> > medium size vacuum desiccator, and pull a vacuum with a high capacity
> > pump, like a dual-stage rotary pump. One used to rough out a large EM
> > column usually works.
> >
> > Phil
> >
> > We're exploring a treatment in which small short-lived (perhaps on the
> > order of seconds) gas bubbles may be generated in tissue. To this
> > end, we're interested in learning the 'instantaneous' state of the
> > tissue immediately after treatment with regard to the presence of any
> > bubbles. Currently, our experiments utilize rabbit muscle. Are there
> > any known histological sample preparation/analysis procedures that
> > have been used to preserve/observe resident gas bubbles? We have
> > considered a flash freezing followed by staining and/or electron
> > microscopy. However, our expertise is not in histology, so we'd very
> > much appreciate any suggestions and/or details regarding possible
> > sample preparation. Thanks.
> > Wayne
> > --
>
>
> /**********************/
>
> /Wayne Kreider/
>
> /University of Washington/
>
> /Applied Physics Lab / CIMU/
>
> /106 Old Fisheries/
>
> /206-543-1324/
>
>
>
> ------------------------------
>
> Message: 10
> Date: Tue, 2 Nov 2004 12:55:07 -0500
> From: tdobersztyn <@t> chmca.org
> Subject: [Histonet] Re: Polyoma Virus
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <OFD41B23A3.1BE9AB68-ON85256F40.006215F7 <@t> chmca.org>
> Content-Type: text/plain; charset=US-ASCII
>
>
>
>
>
>
>
>
> Debra-
> BK, Polyoma virus, can be demonstrated beautifully with Electron
> Microscopy.
> That is, if you have access to an EM lab.
> I am frequently processing renal core biopsies to r/o BK.
> If you would like assistance-
> let me know.
>
>
>
> Date: Mon, 1 Nov 2004 16:16:43 -0500
> From: Browning Deb <browning <@t> HHSC.CA>
> Subject: [Histonet] polyoma virus, SV40
> To: "'histonet <@t> lists.utsouthwestern.edu'"
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
>
> <3AADFB88753AD31189C100902786B91C0E27851F <@t> hch_nt_exchange.hhsc.ca>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Is anyone out there using an antibody against polyoma virus, SV40; BK; or
> JC, against human tissue for demonstrating kidney rejection? If so, could
> you share the details, thanks.
>
> Debra Browning, ART
> Technical Specialist, Immunohistochemistry
> Anatomic Pathology
> Hamilton Health Sciences
> phone: (905) 527-4322 ext 46131
> e-mail: browning <@t> hhs
>
>
>
>
>
> Theresa R Dobersztyn HT ASCP
> Electron Microscopy Laboratory
> Department of Pathology
>
> Akron Children's Hospital
> 1 Perkins Square
> Akron, Ohio 44308
>
> 330-543-8279
>
>
>
>
> ------------------------------
>
> Message: 11
> Date: Tue, 2 Nov 2004 20:06:49 -0500
> From: <lpwenk <@t> sbcglobal.net>
> Subject: Re: [Histonet] AFB Stain
> To: "Etheridge, Sandra AGF:EX" <Sandra.Etheridge <@t> gems8.gov.bc.ca>,
> <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <022801c4c141$65f66ca0$6639d445 <@t> domainnotset.invalid>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Check with your microbiology department. They are probably doing it
> already.
>
> One difference I've found between doing this procedure on smears vs.
> fixed/processed tissue - with smears, there is a 5 minute acid-alcohol
> decolorization. With fixed/processed/paraffin embedded tissue, reduce this
> to 2-3 seconds. The AFB cells walls have been compromised with the
> fixative
> and alcohols and xylene of the processing.
>
> Peggy A. Wenk, HTL(ASCP)SLS
> William Beaumont Hospital
> Royal Oak, MI 48073
>
> ----- Original Message -----
> From: "Etheridge, Sandra AGF:EX" <Sandra.Etheridge <@t> gems8.gov.bc.ca>
> To: <histonet <@t> lists.utsouthwestern.edu>
> Sent: Tuesday, November 02, 2004 12:57 PM
> Subject: [Histonet] AFB Stain
>
>
> > Hello, all,
> >
> > Does anyone have a current method for acid fast bacilli using
> > auramine-rhodamine? Is this a fluorescent stain?
> >
> > Thanks in advance.
> >
> > Sandra Etheridge
> >
> > BC Ministry of Agriculture, Food & Fisheries
> > Animal Health Center, Histology
> > Abbotsford, BC
> > Canada
> >
> > _______________________________________________
> > Histonet mailing list
> > Histonet <@t> lists.utsouthwestern.edu
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Wed, 03 Nov 2004 12:33:41 +0200
> From: Marshall <marshall <@t> cormack.uct.ac.za>
> Subject: [Histonet] Merkel cell staining
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <4188B405.A7723577 <@t> cormack.uct.ac.za>
> Content-Type: text/plain; charset=us-ascii
>
> Hi all
> I am looking to demonstrate Merkel cells in skin. I can not find any
> reference in my textbooks to their demonstration although I have not
> used the internet. Also I have tried silver stains for demoing free
> nerve endings in skin with not much success. The immunocytochemistry for
> neuro filament protein was a bit better but not great. Is there anyway I
> can improve on this?
>
>
>
> ------------------------------
>
> Message: 13
> Date: Wed, 03 Nov 2004 09:41:53 -0500
> From: "Peter Rippstein" <prippstein <@t> ottawaheart.ca>
> Subject: [Histonet] LR White vs LR Gold
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <s188a7f4.031 <@t> mail.ottawaheart.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> Hello Colleagues
>
> Have any of you had the opportunity to compare EM immunogold labelling
> efficiency of LR White vs LR Gold acrylic resins? Protocols would be
> helpful.
> Many thanks.
>
> Peter
>
> Peter Rippstein ART, MLT
> University of Ottawa Heart Institute
> 40 Ruskin St., Room H453A
> Ottawa, Ontario
> Canada, K1Y 4W7
>
> Tel: (613) 761-5282
> Fax: (613) 761-5281
> email: prippstein <@t> ottawaheart.ca
> -------------- next part --------------
> BEGIN:VCARD
> VERSION:2.1
> X-GWTYPE:USER
> FN:Peter Rippstein
> EMAIL;WORK;PREF;NGW:PRippstein <@t> ottawaheart.ca
> N:Rippstein;Peter
> END:VCARD
>
>
> ------------------------------
>
> Message: 14
> Date: Wed, 3 Nov 2004 08:54:40 -0600
> From: Sharon Allen <SAllen <@t> exchange.hsc.mb.ca>
> Subject: [Histonet] RE: Modified Gomori's Trichrome
> To: 'Bruce Abaloz' <brucea <@t> unimelb.edu.au>, "Histonet (E-mail)"
> <histonet <@t> pathology.swmed.edu>
> Message-ID:
> <304B5A264EC9974E8121B0EA14A9C3936619BA <@t> hsc01mx1.hsc.mb.ca>
> Content-Type: text/plain; charset="us-ascii"
>
> Hi Bruce,
> I know the Gomori's Trichrome isn't specific for mitochondria but it
> should
> stain red if the test is working properly. I have been doing this stain
> routinely for 4 different Neuropathologists & am now doing the muscles for
> another Neuropathologist who is apparently an expert in the field &
> extremely fussy. They all are of the same opinion. Having the mitochondria
> stained seems to be imperative for an acceptable stain. Using yet another
> stain on the muscle bx's is not an option. This is why I am attempting to
> find the key to the "perfect Gomori's Trichrome stain". Using the
> celestine
> blue before the Mayer's has worked well for staining the mitochondria (I
> have done 40 of them), but I have no explanation why. My theory is very
> rusty & hoped someone with more knowledge than I could explain the reason
> to
> me. I think it may have something to do with Celestine Blue staining DNA &
> being a oxazine dye. Also chromoptrope 2R being a metachromatic stain.
> I would appreciate any help you can give me.
> Thanks for your reply,
> Sharon
>
> -----Original Message-----
>
> This e-mail and/or any documents in this transmission is intended for the
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>
> ------------------------------
>
> Message: 15
> Date: Wed, 03 Nov 2004 10:32:03 -0500
> From: "Amy Kozer" <alkozer <@t> piadc.ars.usda.gov>
> Subject: [Histonet] Envision System with Vector Red Background
> To: <histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <s188b672.089 <@t> FSNAA-2.naa.ars.usda.gov>
> Content-Type: text/plain; charset=US-ASCII
>
> I've tried using Vector Red with Envision system for permanance and have
> encountered a lot of background and precipitate.
> I've been staining frozen sections of epithelium fixed with acetone.
> Has anyone had any experience with this? Remedies?
>
> Thank you
> Amy Kozer
> PIADC, ARS, USDA
>
>
>
> ------------------------------
>
> Message: 16
> Date: Wed, 03 Nov 2004 10:52:46 -0500
> From: "Richard Cartun" <Rcartun <@t> harthosp.org>
> Subject: Re: [Histonet] Merkel cell staining
> To: <marshall <@t> cormack.uct.ac.za>,<histonet <@t> lists.utsouthwestern.edu>
> Message-ID: <s188b88d.075 <@t> harthosp.org>
> Content-Type: text/plain; charset=US-ASCII
>
> Try IHC for cytokeratin 20.
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> >>> Marshall <marshall <@t> cormack.uct.ac.za> 11/03/04 05:33AM >>>
> Hi all
> I am looking to demonstrate Merkel cells in skin. I can not find any
> reference in my textbooks to their demonstration although I have not
> used the internet. Also I have tried silver stains for demoing free
> nerve endings in skin with not much success. The immunocytochemistry
> for
> neuro filament protein was a bit better but not great. Is there anyway
> I
> can improve on this?
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 17
> Date: Wed, 03 Nov 2004 11:09:31 -0500
> From: Rita Angel <RITA.ANGEL <@t> UC.EDU>
> Subject: [Histonet] B & D steamer
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <5.1.0.14.2.20041103110800.00b24e08 <@t> ucmail3.uc.edu>
> Content-Type: text/plain; charset="us-ascii"; format=flowed
>
> Thanks to everyone for their input on the type of containers you use in
> the
> steamer. I'll try the plastic coplin jars!!
>
> Thanks again,
>
> Rita Angel HT (ASCP)
>
>
>
>
> ------------------------------
>
> Message: 18
> Date: Wed, 3 Nov 2004 08:52:46 -0800 (PST)
> From: Histology SLU <sluhisto <@t> yahoo.com>
> Subject: [Histonet] Polyoma BK virus controls
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <20041103165246.4952.qmail <@t> web51001.mail.yahoo.com>
> Content-Type: text/plain; charset=us-ascii
>
> Does anyone have BK virus controls that you would be willing to trade for
> something we may have that you would need? Any help in locating control
> blocks or slides would be greatly appreciated. Thanks.
>
> Susan
>
>
> ---------------------------------
> Do you Yahoo!?
> Check out the new Yahoo! Front Page. www.yahoo.com/a
>
> ------------------------------
>
> Message: 19
> Date: Wed, 3 Nov 2004 11:46:59 -0600
> From: "Grant, Debra" <DRG <@t> Stowers-Institute.org>
> Subject: [Histonet] BMP7
> To: <histonet <@t> pathology.swmed.edu>
> Message-ID: <1112618780-192831059 <@t> pathology.swmed.edu>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Has anyone used BMP7 antibody on mouse? If so, could you please send me
> all the information, company, catalog #, protocol etc.
>
> Thanks in advance!
>
> Debby Grant
> Research Technician II
> Histology Core Facility
> Stowers Institute for Medical Research
> 1000 E. 50th Street
> Kansas City, MO 64110
> drg <@t> stowers-institute.org
>
>
>
>
> ------------------------------
>
> Message: 20
> Date: Wed, 03 Nov 2004 09:52:01 -0800
> From: "Ant S." <neuroant <@t> hotmail.com>
> Subject: [Histonet] Thanks for the Teasing
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID: <BAY15-F22ikaTAbNF440001aced <@t> hotmail.com>
> Content-Type: text/plain
>
>
> Thanks for the great tips on Nerve Teasing. I have quite a few good
> ideas to experiment with now. I appreciate the help. This is a very
> informative community (and mostly cheerful, too!)
>
> Thanks,
>
> Antoinette Swensson
> Univeristy of Washington/Harborview Medical Center
> Neuropathology
> _________________________________________________________________
>
> [1]Find the music you love on MSN Music. Start downloading now!
>
> References
>
> 1. http://g.msn.com/8HMBENUS/2731??PS=47575
>
>
> ------------------------------
>
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>
> End of Histonet Digest, Vol 12, Issue 4
> ***************************************
>
>
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