[Histonet] RE: Modified Gomori's Trichrome

[Sharon Allen]

Hi Bruce,
I know the Gomori's Trichrome isn't specific for mitochondria but it should
stain red if the test is working properly. I have been doing this stain
routinely for 4 different Neuropathologists & am now doing the muscles for
another Neuropathologist who is apparently an expert in the field &
extremely fussy. They all are of the same opinion. Having the mitochondria
stained seems to be imperative for an acceptable stain. Using yet another
stain on the muscle bx's is not an option.  This is why I am attempting to
find the key to the "perfect Gomori's Trichrome stain". Using the celestine
blue before the Mayer's has worked well for staining the mitochondria (I
have done 40 of them), but I have no explanation why. My theory is very
rusty & hoped someone with more knowledge than I could explain the reason to
me. I think it may have something to do with Celestine Blue staining DNA &
being a oxazine dye. Also chromoptrope 2R being a metachromatic stain.
I would appreciate any help you can give me. 
Thanks for your reply,
Sharon

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