[Histonet] AFB Stain

[lpwenk <@t> sbcglobal.net]

Check with your microbiology department. They are probably doing it already.

One difference I've found between doing this procedure on smears vs.
fixed/processed tissue - with smears, there is a 5 minute acid-alcohol
decolorization. With fixed/processed/paraffin embedded tissue, reduce this
to 2-3 seconds. The AFB cells walls have been compromised with the fixative
and alcohols and xylene of the processing.

Peggy A. Wenk, HTL(ASCP)SLS
William Beaumont Hospital
Royal Oak, MI 48073

----- Original Message -----
From: "Etheridge, Sandra AGF:EX" <Sandra.Etheridge <@t> gems8.gov.bc.ca>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Tuesday, November 02, 2004 12:57 PM
Subject: [Histonet] AFB Stain


> Hello, all,
>
> Does anyone have a current method for acid fast bacilli using
> auramine-rhodamine?  Is this a fluorescent stain?
>
> Thanks in advance.
>
> Sandra Etheridge
>
> BC Ministry of Agriculture, Food & Fisheries
> Animal Health Center, Histology
> Abbotsford, BC
> Canada
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet