[Histonet] 1) steaming slides 2) expired reagents for IHC

[Terri Thinnes]

1) I use plastic slide mailers filled with the antigen retrieval solution (about 15 to 25 ml) and place it in a beaker. Neither one have cracked or melted in the steamer.

2) I have had a few experiences with reagents/kits failing before their expiration date so I  regard the expiration date as a guide only. I sent the kit back to the supplier and they always "verified" the failure and sent me a new one but a lot of time and frustration was involved.