[Histonet] RE: p21 mAb

[C.M. van der Loos]

Dear Richard,
For p21 WAF/CIP1 we used DakoCyto antibody M7202, clone SX118. After HIER (Tris-EDTA pH9.0, 15 min 100C) the antibody stained very well at 1:25 (60 min, RT) using an EnVision+ detection with DAB+ as chromogen. As "counterstain" we performed double staining with CD31 and alpha-actin to get an idea which nuclei actually stained. Results were great when using sequential double staining: p21 - EnV+ - DAB+ - CD31 or SMA - PowerVision-GAM/AP - Liquid Permanent Red (not too dark!!) - haematoxilin (weak!!). 
Hope this helps.

Chris van der Loos, PhD
Dept. of Pathology
Academic Medical Center
Amsterdam - The Netherlands  

----- Original Message ----- 
>From  "Richard Cartun" <Rcartun <@t> harthosp.org> 
Date  Mon, 01 Nov 2004 10:23:41 -0500 
To  <histonet <@t> lists.utsouthwestern.edu> 
Subject  [Histonet] p21 mAb 
Is anyone doing IHC for p21 (clone EA10) on paraffin sections?  If so,
where do you get it?  Thank you!

Richard


Richard W. Cartun, Ph.D.
Director, Immunopathology & Histology
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-0174 Fax