[Histonet] exotic species/chief&parietal cells

[Margaret Blount]

Dear Sharon,

I remembered Harry Cook when you posted your question. He always had very
good transparencies when he gave his lectures and I thought he showed us
sections of just what you are looking for so I had a look through his
book:Histological Demonstration Techniques. Here are the methods he
mentions:

PAS-Toluidine Blue-Aurantia (Cook, 1962, Stain Technology 37, 317) and
Methasol Fast Blue-PAS-Alcian Yellow (Maxwell, 1963, Stain Technology, 38,
286). I haven't tried these, but if Cook recommended them they should be OK.

I attach the methods, but I don't know if you can still get all the
components.


Good luck anyway.

Best wishes

Margaret

Margaret Blount
Chief Technician
Clinical Biochemistry
University of Cambridge
Addenbrooke's Hospital
Hills Road
Cambridge
CB2 2QR 



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of Marshall
Sent: Wednesday, May 26, 2004 11:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] exotic species/chief&parietal cells


Hi all
We are looking at the gastric mucosa of the Cape Mole Rat and can not
distinguish chief cells on the basis of morphology. There seem to be
more parietal cells and we can not be sure of the chief cells. Is there
a stain we can use to distinguish between these to cell types as the
Hematoxylin & Eosin stain is not making this obvious. 
Thanks
Sharon Marshall
Dept. Human Biology
University of Cape Town
South Africa
e-mail: marshall <@t> cormack.uct.ac.za

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