[Histonet] Osmium Tetroxide Protocol
asmith <@t> mail.barry.edu
Wed May 26 08:35:19 CDT 2004
Osmium tetroxide is a gas, usually sold dissolved in water. It has a nasty
tendency to evaporate from the solution. It reacts instantly with amino
alcohols (cell membranes) and with cytokeratins (skin and cornea) to leave
black deposits of osmium metal. Deposits in the cornea interfere with
vision for many weeks. You must use it in a fume hood that draws really
well. In one of my postdoctoral positions we didn't trust our fume hood and
used osmium tetroxide outdoors only on days when the wind speed was 15 mph
or greater. One, of course, was careful to stay upwind of one's work!
Goggles or wrap-around safety glasses are also essential in case the #@$*
Allen A. Smith, Ph.D.
Professor of Anatomy
School of Graduate Medical Sciences
Podiatric Medicine and Surgery
Miami Shores, Florida
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
mprice26 <@t> juno.com
Sent: Tuesday, May 25, 2004 3:07 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Osmium Tetroxide Protocol
When handling Osmium Tetroxide, is a chemical fume gard such as an Bench Top
fume absorber appropriate to use for this chemical?
Also, should you wear other PPE besides gloves, gown etc. Should you wear a
The best thing to hit the Internet in years - Juno SpeedBand!
Surf the Web up to FIVE TIMES FASTER!
Only $14.95/ month - visit www.juno.com to sign up today!
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
The information transmitted is intended only for the person or entity to which it is addressed and may contain confidential, and/or privileged material. No confidentiality or privilege is waived or lost by any errant transmission. If you receive this message in error, please immediately delete it and all copies of it from your system and notify the sender. E-mail transmission cannot be guaranteed to be secure or error-free as information could be intercepted, corrupted, lost, destroyed, arrive late or incomplete, or contain viruses.
Barry University - Miami Shores, FL (http://www.barry.edu)
More information about the Histonet