[Histonet] Spleen autofluorescence

Helen Newbery h.newbery <@t> ed.ac.uk
Tue May 25 04:14:26 CDT 2004


Hi histonetters,

Apologies if my query is really basic but I am wanting to do immunofluorescent
antibody staining on mouse spleen sections (paraffin-embedded) and am seeing a
lot of autofluorescence, which I presume is due to platelets. Is there any way
around this problem?

Helen Newbery,
Medical Genetics Section,
Molecular Medicine Centre,
Western General Hospital,
Edinburgh,
EH4 2XU.
0131 651 1047




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