[Histonet] Formalin fixation of cells for paraffin embedding?

Eva C. Andersson eca9 <@t> georgetown.edu
Thu May 20 14:18:26 CDT 2004


Hi everyone,
My lab wants to paraffin embed some cells to be used as positive 
controls for immunohisochemistry staining.
We have a cell line growing but need a good protocol for fixing the 
cells with formalin. Once the cells have been fixed the pellet will be 
embedded in paraffin by our histopathology department.

Does anyone have a good and easy method for the fixation part?

We will deliver the pellet to the histopathology department in 70% ethanol.

Thanks in advance,

Eva Andersson
Research Assistant
Georgetown University






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