[Histonet] EGRF

[Patti Loykasek]

Sharon - we have seen this staining in liver (we are using a different clone
of EGFR). You can see staining in normal colon & on nerves, too.

Patti Loykasek




> Histonetters,
> Im in the validation process for EGFR.  I have noticed that the normal liver
> cells in liver core biopsies have a positive membrane stain, 2+ - 3+ .  The
> metastic tumor cells are not difficult to identify, since their membrane
> staining when positive is not nearly as uniform or as intense as the normal
> liver cells.  Has anyone else seen this?  No other normal cells in the tissues
> we have stained  stains  positive.  We are using Ventana antibody on the
> Benchmark and following the recommend protocol.
> 
> Sharon
> Baptist Hospital of E TN
> Knoxville, TN
> 865 549-4351
> 
> 
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