[Histonet] EGRF

[Sebree Linda A.]

Are you using AB block, Sharon?  You may need to block biotin off line also since VMS' AB block is not real strong.

Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Clinical & Research Laboratory
DM223-VA
600 Highland Ave.
Madison, WI 53792
(608)265-6596
FAX:  (608)262-7174



-----Original Message-----
From: E Sharon Shields [mailto:eshields <@t> bhset.org]
Sent: Thursday, May 20, 2004 12:28 PM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] EGRF


Histonetters,
Im in the validation process for EGFR.  I have noticed that the normal liver cells in liver core biopsies have a positive membrane stain, 2+ - 3+ .  The metastic tumor cells are not difficult to identify, since their membrane staining when positive is not nearly as uniform or as intense as the normal liver cells.  Has anyone else seen this?  No other normal cells in the tissues we have stained  stains  positive.  We are using Ventana antibody on the Benchmark and following the recommend protocol.

Sharon
Baptist Hospital of E TN
Knoxville, TN
865 549-4351


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