[Histonet] H and E

Wed May 19 10:43:57 CDT 2004

Ah Barry - life has become instant everything.
Why look longingly at immature H on a windowsill for months when in a flash of periodate, it can be yours, ready, now?

My first experience with Ehrlich's, apart from school, was in my final professional exam in Liverpool, where everything was stained with it. I found it so difficult with all the mucin and ground substance (still mucin I know) staining blue.
Particularly irksome when I like my mucin green:-)

I have always found Gill's to be much as Harris' but more reliable, at least, if you make it yourself.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: Barry R Rittman [mailto:Barry.R.Rittman <@t> uth.tmc.edu]
Sent: 19 May 2004 16:19
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H and E

I personally do not like either Harris or Gill's hematoxylin as I was
brought up using hand staining and Ehrlich's hematoxylin made from
scratch. There are a lot of individuals using both these solution in
Histoland, so I have to assume that they can't all be wrong. There have
been a lot of hematoxylins formulated by individuals from several
cultures (not just dead white guys), after all hematoxylin was used in
South America for dyeing cloth way before anyone thought of using it for
staining tissue sections.
Recently Placido Domingo, who I think we can all agree is a great tenor,
when being interviewed on TV said "I do not understand rap but that is
my problem, I do respect it". I think that this is a valuable lesson
from a great master. 
Let us not knock the younger generation just because they do not
necessarily have our principles or standards. It is arrogant of us to
regard ours as being the only ones possible or desirable. I do believe
that it is common sense to foster innovation. I am 65 but I find that I
can always learn something from "the new generation", life before 30 is
not necessarily wasted and life does not end after 30. 
Barry

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Connie
McManus
Sent: Wednesday, May 19, 2004 8:49 AM
To: 'Gary Gill'; 'Marshall Terry Dr,Consultant Histopathologist'; 'Petia
P Stefanova'; 'Megan Kear'; histonet <@t> lists.utsouthwestern.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems

Gary
Is Gill a relative or yours??? I don't give a hoot who made what when. I
like Harris. I don't believe Gill has made any significant improvements
over the old dead white guy's hematoxylin. 
BTW, classical music may have been composed by dead white guys, but I
don't hear ANYTHING being composed today that has the depth, complexity
and color of those great composers. I've studied piano from since I was
4 yrs old, and I've studied the organ (I've played as a church
organist)for almost 20 years. I know music like the back of my hand and
I love ALL music (except gangstah stuff). SO DON'T try to tell me about
what music is living and worth my while. 
Dead white Europeans. Yeah, right. I'd like to see someone between 8 and
14 in this generation compose music like Felix Mendelsohnn's or
Mozart's. 
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone: 435/797-1891
fax: 435/797-2805

-----Original Message-----
From: Gary Gill [mailto:garygill <@t> dcla.com] 
Sent: Tuesday, May 18, 2004 10:04 AM
To: 'Connie McManus'; 'Marshall Terry Dr,Consultant Histopathologist';
'Petia P Stefanova'; 'Megan Kear'; histonet <@t> lists.utsouthwestern.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems
You may have heard that classical music was composed by dead white
Europeans. Well, Harris hematoxylin was composed by a dead white
American (physician at Jefferson Hospital in 1904). Gill hematoxylin was
composed by a live white American. So if you want to liven things up, go
with Gill's!
Gary Gill (one and the same)
PS -- No royalties involved, thanks to bad advice in 1972 from corporate
counsel for Johns Hopkins Medical School.
-----Original Message-----
From: Connie McManus [mailto:convmcm <@t> cc.usu.edu] 
Sent: Tuesday, May 18, 2004 10:41 AM
To: 'Marshall Terry Dr,Consultant Histopathologist'; 'Petia P
Stefanova'; 'Megan Kear'; histonet <@t> lists.utsouthwestern.edu
Cc: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E stain problems
Wow. What a lot of interesting comments!! 
I agree with Terry re the agitation. When I watch the stainer do those
dips (I can program how many, but NOT the briskness), I wonder if you
could even call it agitation. My hand dips are very brisk. Also, I don't
bother letting the slides stay in the alcohols for 1 minute or 2, I give
the slides about 20 -30 good brisk dips in each solution, then the timed
rinses & staining. This has always been far more satisfactory to me than
those sllllooooowwwww dips from the machine.
As for the kind of hematoxylin, someone suggested I throw out the Harris
and do Gills III. I've tried Gill III before and I much prefer the
Harris. So it's just a matter of personal preference on that... AND what
your pathologist likes *G*
Everyone having an nice Tuesday??? *g*
Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone: 435/797-1891
fax: 435/797-2805

-----

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