[Histonet] Background Staining

[Smith, Emily]

Hello All,
In the course of a recent experiment, I saw strong background staining
on charged slides with frozen cells in OCT (optimal cutting temperature)
when treated with streptavidin-HRP. 
The staining appears over the entire glass surface not only where the
cells and OCT were.  This all-over-staining also appears on clean
(without OCT on them) slides that are processed with the OCT slides.  I
have tried extensive water washes and the results remain the same.  No
primary antibody is added and the results are the same.
Have you seen this before? Do you have any suggestions? 
Thank you for your help.
~Emily

Emily A. Smith
CuraGen Corporation


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