[Histonet] Congo Red

Karen Wittler kwittle <@t> jhmi.edu
Tue May 18 15:29:47 CDT 2004

 We stain our Congo Reds on an automated stainer now. Before that we
stained with hematoxylin first because rinsing and differentiation was
required and we used an alkaline Congo Red. After staining with the
Congo Red in a saturated sodium chloride- alcohol solution,we did not
rinse,but rapidly dehydrated, cleared and mounted 

 For our H&Es we use Modified Harris Hematoxylin and Eosin-Y w/
Phloxine both manufactured by Richard Allen.

Karen Wittler, HT(ASCP)
Johns Hopkins Hospital
Baltimore, MD

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