[Histonet] Fixation and Decalcification of Bone Marrow Bxs.

[Rena Fail]

We use formic acid for decalcification. It's slow, but the morphology is
preserved and the results with IHC are excellent.
Rena Fail HT(ASCP)QIHC
Lead Tech IHC/SS
MUSC

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Henry
Charlene
Sent: Friday, May 14, 2004 4:34 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fixation and Decalcification of Bone Marrow Bxs.


Fellow Histotechs,
I would like to get some feedback on fixatives and decalcification
protocols used in your laboratories. Our pathologists, like many, want
perfection; so here is my problem. We were fixing our bone marrow bxs.
in 10% NBF and then decalcification for 1 hour prior to processing.
Because of the facility I work at, we perform IHC and ISH on a large
percentage of our bxs. With the method we were using, some of our
antibodies would not work on tissue that had been decalcified and the
decalcification completely destroyed the RNA making it impossible to
perform ISH. About 6 months ago, we switched to fixing our bone marrows
in 10% NBF saturated with EDTA (this fixes and decals at the same time)
with beautiful results with our IHC and also ISH. Even though our
pathologists are elated that the IHC and ISH are great, they are not
completely happy with the morphology of the H&E slides. I was just
wondering if anyone has any suggestions so we can accomplish both great
H&E morphology and great stains with IHC and ISH. 
Thanks,

Charlene Henry, HT QIHC
Histology/IHC Section Head
Department of Pathology
St. Jude Children's Research Hospital
332 North Lauderdale
Memphis, TN  38105
Phone: 901-495-3349
FAX: 901-495-3100


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