[Histonet] cytochrome oxidase

[Geoff McAuliffe]

Dear Luz:

Luz Cardenas wrote:

>Hi everyone,
>1.Can cytochrome oxidase method stain fixed tissue (4% Paraformaldehyde)?
>
Yes, provided the fixation is short. Very short. Fix fresh frozen 
sections 5 min in cold buffered formalin. Whole animal perfusion for 15 
seconds followed by a buffered sucrose washout in preparation for frozen 
sections.

>2. Is there any specific method for fixed tissue?
>
Lots of published methods. See John Kiernan's book (which you should 
have in the lab) or papers by Margaret Wong-Riley. I don't know if the 
"standard" histopath texts would have this method, perhaps too specialized?

Geoff

>
>Thank you very much in advance!
>Luz.
>
>Luz Cárdenas, MD
>Department of Anatomy and Neurobiology
>University of Tennessee Health Science Center
>855 Monroe Avenue, Room 309/302
>Memphis, TN 38163
>Lab: (901) 448-6002 / 7318
>Fax: (901) 448-7193 (Dept)
>lcardenas <@t> utmem.edu
>
>
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>  
>

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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029 
mcauliff <@t> umdnj.edu
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