[Histonet] Breast processing

[Marshall Terry Dr, Consultant Histopathologist]

" You can bet if it was their wife they would insist on the tissue being fixed longer."

I doubt it. 
Most pathologist and some techs from this list, think that a tissue is fixed when it's not bloody any more.

Dr Terry L Marshall, B.A.(Law), M.B.,Ch.B.,F.R.C.Path
 Consultant Pathologist
 Rotherham General Hospital
 South Yorkshire
 England
        terry.marshall <@t> rothgen.nhs.uk

-----Original Message-----
From: Horn, Hazel V [mailto:HornHV <@t> archildrens.org]
Sent: 12 May 2004 15:46
To: Bryan Hewlett; GREYTRUNK <@t> aol.com; browning <@t> HHSC.CA;
mary.bliss <@t> northwestpathology.com; histonet <@t> pathology.swmed.edu
Subject: RE: [Histonet] Breast processing


Thank you Bryan for pointing this out.   As a BC survivor it means a lot
to me to know there are labs who are following proper protocol.    I
understand it's difficult to get pathologists to wait the proper time
for fixation of breast tissue but techs must demand this.   You can bet
if it was their wife they would insist on the tissue being fixed longer.


Hazel Horn, HT/HTL (ASCP)
Histology Supervisor
Arkansas Children's Hospital

Phone - 501.364.4240
Fax - 501.364.3912 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bryan
Hewlett
Sent: Tuesday, May 11, 2004 7:51 PM
To: GREYTRUNK <@t> aol.com; browning <@t> HHSC.CA;
mary.bliss <@t> northwestpathology.com; histonet <@t> pathology.swmed.edu
Subject: Re: [Histonet] Breast processing


Just another FYI-----

Fixation of breast tissue for the IHC demonstration of HER2 requires at
least 16-24 hours in NBF. 4 hours in NBF on the processor comes nowhere
near the FDA approved protocol for HER2, unless your tissue has already
spent at least 12 hours in NBF prior to being placed on the processor!!

Bryan

----- Original Message -----
From: <GREYTRUNK <@t> aol.com>
To: <browning <@t> HHSC.CA>; <mary.bliss <@t> northwestpathology.com>;
<histonet <@t> pathology.swmed.edu>
Sent: Tuesday, May 11, 2004 7:40 PM
Subject: Re: [Histonet] Breast processing


> Just an FYI----
> All of those labs that is using the FDA approved protocol for Her2 
> should only be using formalin on the processors and for fixation.  We 
> use the
following
> protocol and it works great.
>
> 10% NBF 2 hours
> 10% NBF 2 hours
> 70% ethanol 45 minutes
> 80% ethanol 45 minutes
> 95% ethanol 45 minutes
> 100% ethanol 45 minutes
> 100% ethanol 45 minutes
> 100% ethanol/xylene mix 45 minutes
> xylene 45 minutes
> xylene 45 minutes
> paraplast 30 minutes
> paraplast 30 minutes
> paraplast 30 minutes
> paraplast 30 minutes
>
> This protocol works great on fatty tissue as well, but again the 
> section
size
> must be reasonable ( no more than 4 mm)
> Roxanne
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> Histonet <@t> lists.utsouthwestern.edu 
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>



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