[Histonet] Alternative's to the Steiner

[GUTIERREZ, JUAN]

I'm not sure about the Steiner, but we used phosphomolybdic acid one time with our Dieterle stain.  The concentration eludes me, but I think it was something like 5 or 10 percent.  Good luck.

Juan C. Gutierrez, HT(ASCP)
Histology Supervisor
Christus Santa Rosa Hospital
333 N. Santa Rosa Ave.
San Antonio, TX  78207


-----Original Message-----
From: Bonner, Janet [mailto:Janet.Bonner <@t> FLHOSP.ORG]
Sent: Wednesday, May 12, 2004 9:43 AM
To: 'Mary Reeves'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Alternative's to the Steiner


Speaking of the Steiner, is there a substitute solution that can be used
instead of 1% Uranyl Nitrate?  I would appreciate any help - Thank you.
Janet



-----Original Message-----
From: Mary Reeves [mailto:REEVEML <@t> shands.ufl.edu]
Sent: Tuesday, May 11, 2004 8:11 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Alternative's to the Steiner


I have been given alternatives's to stain Helicobacter pylori, however,
what about the other organisms stained by the Steiner method?
-spirochetes
-legionella
-donavan bodies
-cat scratch



Mary Reeves
Technical Specialist
Histology
1-352-265-0680 ext 7-2113

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