[Histonet] Rta Brain - processing

[balajimr <@t> drreddys.com]

Can somebody suggest me a best processing for rat brains. Recently I am 
having the problem of cracks and tares in the brain sections. We are 
collecting the sections at 40 degree c. Any suggestions are welcome. 
Thanks in advance. Following is my processing schedule:


 Station No.
Solvent
Time (Hr)
1
Isopropyl Alcohol 80%
1
2
Isopropyl Alcohol 90%
1
3
Isopropyl Alcohol 95%
1
4
Isopropyl Alcohol 95%
1
5
Isopropyl Alcohol 100%
1
6
Isopropyl Alcohol 100%
1
7
Isopropyl Alcohol 100%
1
8
Chloroform
1
9
Chloroform
1
10
Chloroform
1
11
Paraffin I
3
12
Paraffin II
3