[Histonet] Breast processing

[Bryan Hewlett]

Just another FYI-----

Fixation of breast tissue for the IHC demonstration of HER2 requires at
least 16-24 hours in NBF.
4 hours in NBF on the processor comes nowhere near the FDA approved protocol
for HER2, unless your tissue has already spent at least 12 hours in NBF
prior to being placed on the processor!!

Bryan

----- Original Message -----
From: <GREYTRUNK <@t> aol.com>
To: <browning <@t> HHSC.CA>; <mary.bliss <@t> northwestpathology.com>;
<histonet <@t> pathology.swmed.edu>
Sent: Tuesday, May 11, 2004 7:40 PM
Subject: Re: [Histonet] Breast processing


> Just an FYI----
> All of those labs that is using the FDA approved protocol for Her2 should
> only be using formalin on the processors and for fixation.  We use the
following
> protocol and it works great.
>
> 10% NBF 2 hours
> 10% NBF 2 hours
> 70% ethanol 45 minutes
> 80% ethanol 45 minutes
> 95% ethanol 45 minutes
> 100% ethanol 45 minutes
> 100% ethanol 45 minutes
> 100% ethanol/xylene mix 45 minutes
> xylene 45 minutes
> xylene 45 minutes
> paraplast 30 minutes
> paraplast 30 minutes
> paraplast 30 minutes
> paraplast 30 minutes
>
> This protocol works great on fatty tissue as well, but again the section
size
> must be reasonable ( no more than 4 mm)
> Roxanne
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