[Histonet] Research Diagnstics CD 66

[Melanie Black]

Hi There

I am running an antibody from Research Diagnostics - CD 66 
(Granulocyte Marker).Has anybody had success with it on formalin 
fixed tissue, and is so, what antigen retrieval have you found to be 
successful. I have had no problem with it on unfixed blood smears, 
BUT no luck on fixed tissue.

My antibody is RDI-CD66B-G10F5, and is recommended for formalin fixed 
tissue, with citrate pre treatment. It also requires an IgM 
secondary, which I have used!I have tries citrate buffer as well as 
trypsin.

Any suggestions???

Many Thanks
Melanie Black.
-- 
Cardiovascular Research Unit
Div. of Cardiothoracic Surgery
Chris Barnard Building
University of Cape Town
Anzio Road
Observatory
7925
Republic of South Africa

Tel +27 21 406-6589
Cel +27 82 469-3352
Fax +27 21 448-5935