[Histonet] Help - Zebra Fish

[Connie McManus]

I'm not an expert in fish histology, but embryos, being very fragile,
would be best processed with minimal times.  I'm assuming you will be
doing these in paraffin.  After being properly fixed, I would process
them, in ethanol, 70%, 80%, 95%, absolute, absolute + xylene, xylene (2
changes / 15 minutes each), then paraffin, 58 C, 4 changes, 30 minutes
each change. I prefer xylenes, not the substitutes, but if that's what
you use... I use this protocol for mouse livers and any other small,
fragile tissues that come through here and it works wonderfully.  

Hope this helps

Connie McManus
Utah Veterinary Diagnostics Laboratory
Utah State University
Logan, UT
Phone:  435/797-1891
fax: 435/797-2805


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of jo-ann
Sent: Monday, May 10, 2004 7:32 AM
To: Histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Help - Zebra Fish

Hi Everyone,

My new challenge for this week is that someone has asked me to process
Zebra
Fish embryos.   I haven't got a clue of a processing schedule.  Can
someone
help please.  I am meeting with the  Investigator tomorrow.

Thanks in advance

Jo-Ann Bader


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