[Histonet] cutting rat pancreas

[Margaret Blount]

You mention a glycerin solution, what concentration of glycerin? All these
tips are very helpful.

Thanks

Margaret

Margaret Blount
Chief Technician
Clinical Biochemistry
University of Cambridge
Addenbrooke's Hospital
Hills Road
Cambridge
CB2 2QR 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu]On Behalf Of
KAELPERS <@t> aol.com
Sent: Friday, May 07, 2004 4:24 AM
To: pstefanova <@t> sten.sunnybrook.utoronto.ca;
histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] cutting rat pancreas


Pancreas tissue is always a problem...we have tried cutting larger sections,

blocking them with other tissue, and they always seem to be a lot drier.  We

just block them with intestines and let them soak on ice water to soften.
Also 
you can soak them in a glycerin solution this tends to soften the tissue for

good sectioning.....our techs swear by it.

lge
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