[Histonet] re mounting media for NBT/BCIP

Carl carl.hobbs <@t> kcl.ac.uk
Mon May 3 13:01:22 CDT 2004


Why an aq. one? Thoroughly air-dry, soak in xylene for 10mins, then mount in
DPX? Best resolution. Aq. mountants don't give as good a resolution, partic.
when preparing photomicrographs/using high power.
Best aq. mountants for me: gelatin-based ones eg Gelvatol, that set at RT,
or Mowiol-based mountant that set, too( yes, it is designed for fluorescent
dyes)


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