[Histonet] Lectin IHC

[Amrit Samra]

I am doing lectin staining for frozen guinea pig tissues. I have tried
different fixitives; bouin, 10% formalin, acetone, ethanol, B5 and
different buffers; TBS, PBS and lectin buffer. I am still not able to
get a positive staining for my known positive. The last trial I did was
fixing in bouin and washing with TBS.
any help would be greatly appreciated.

Jenny



Amrit Samra
Histology Lab,the iCAPTURE Centre
St.Paul's Hospital 
1081 Burrard Street,Vancouver,BC
Canada V6Z 1Y6
Phone: 604-682-2344 extension 62703