[Histonet] Apoptosis detection -Caspase 3-

[Serrano del Pozo, Erika]

Hi Jackie,

As Joelle, I am looking for an ICC technique to detect apoptosis cells,
instead mine are muscular cells (C2C12). 

I am really interested in the Caspase 3 antibody. Could you tell me which
commercial antibody do you use and the protocol to perform the ICC ( I´m
used to perform IHC not ICC, and I´m not really sure if there is any "triky"
step). 

Thanks in advance.


> Erika Serrano
> 
> Centre de Regulació Genòmica
> Differenciation and Cancer Programme
> Barcelona -SPAIN-  
> 
> 
> ----------
> From: 	Jackie.O'Connor <@t> abbott.com
> Sent: 	Tuesday, March 30, 2004 15:06 PM
> To: 	Joelle Alcock
> Cc: 	histonet <@t> lists.utsouthwestern.edu
> Subject: 	Re: [Histonet] Apoptosis detection
> 
> The Caspase 3 antibody demonstrates only apoptotic cells. 
> 
> Jackie O'Connor
> Abbott Laboratories
> Abbott Park, IL
> 
> 
> 
> 
> 
> 
> 
> 
> "Joelle Alcock" <plxja <@t> nottingham.ac.uk>
> Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
> 03/29/2004 03:51 PM
> 
>  
>         To:     <histonet <@t> lists.utsouthwestern.edu>
>         cc: 
>         Subject:        [Histonet] Apoptosis detection
> 
> 
> Hi all
> 
> I am looking for an IHC technique to detect apoptosis cells on testis 
> sections. I have previously used the Tunnel assay but would like an 
> alternative as often meiotic cells are also labelled and I want to avoid 
> this.
> 
> I have seen the Histone labelling assay by UPSTATE but I think that is 
> mainly for ICC not IHC.
> 
> Thanks for any help
> 
> Joelle Alcock
> 
> Institute of Genetics
> Queens Medical Centre
> Nottingham, UK
> 
> 
> 
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