[Histonet] Fixation for lectin work

[Barry R Rittman]

Pablo
Bouin's fixative can be a good fixative for some IHC applications but I cannot agree that it is good for lectin studies.
I did some studies on lectin binding severl year ago and found that the best fixative to be alcohol and acetone. Alcohol acetic probably is OK too. 
An important point here however is that there is considerable variation depending on the tissue and also on the lectin that is used.
Barry

	-----Original Message----- 
	From: histonet-bounces <@t> lists.utsouthwestern.edu on behalf of Robert Schoonhoven 
	Sent: Tue 3/30/2004 1:19 PM 
	To: Pablo Sánchez Quinteiro 
	Cc: Histonet <@t> lists.utsouthwestern.edu 
	Subject: Re: [Histonet] Fixation for lectin work
	
	

	Must be more to it then that.  Bouin's is made up with formaldehyde.
	
	Robert Schoonhoven
	
	Pablo Sánchez Quinteiro wrote:
	
	>I do not know the reason, but I remember that few years ago that fact
	>caused me chronic headache. Just after switching to Acetic-Alcohol fixed
	>material I got nice labelling. Bouin fluid is also an excellent fixative
	>for lectin studies.
	>
	>Sometimes aldehydes work in some tissues, so before working with lectins it
	>is advisable to compare different fixatives.
	>
	>Cheers
	>
	>Pablo Sanchez
	>
	>At 12:04 p.m. 30/03/04 -0600, you wrote:
	> 
	>
	>>Dear Histonetters:
	>>
	>>Does anyone know why 10% buffered formaldehyde may not be suitable for
	>>lectin labeling?
	>>
	>>John C. Dennis
	>>
	>>
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