[Histonet] Apoptosis detection

[Bryan Hewlett]

Joelle,

Apoptosis is apoptosis and may well be part of the meiotic process.
You may wish to consider APAF1 or the one of the caspase antibodies from
Novacastra as an alternative to TUNEL.
You could also check with Neil Hand in the pathology IHC lab at Queens for
his recommendation.

Regards,

Bryan


----- Original Message -----
From: "Joelle Alcock" <plxja <@t> nottingham.ac.uk>
To: <histonet <@t> lists.utsouthwestern.edu>
Sent: Monday, March 29, 2004 4:51 PM
Subject: [Histonet] Apoptosis detection


Hi all

I am looking for an IHC technique to detect apoptosis cells on testis
sections. I have previously used the Tunnel assay but would like an
alternative as often meiotic cells are also labelled and I want to avoid
this.

I have seen the Histone labelling assay by UPSTATE but I think that is
mainly for ICC not IHC.

Thanks for any help

Joelle Alcock

Institute of Genetics
Queens Medical Centre
Nottingham, UK



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