[Histonet] Goodpasture's carbol fuchsin stain

[Bartlett, Jeanine]

Also in Sheehan's.

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bryan
Llewellyn
Sent: Wednesday, March 24, 2004 3:40 PM
To: Histonet; Kathleen Cormier
Subject: Re: [Histonet] Goodpasture's carbol fuchsin stain


It is in Lillie's book as the Goodpasture-Perrin method for influenza
organisms, encephalitozoa and toxoplasmata (Arch. Path. 36:568, 1943)

Carbol aniline fuchsin:
    Basic fuchsin 0.59 g
    30% alcohol 100 mL
    Analine 1 mL
    Phenol 1 g

Fix in Zenker or Orth, or formalin fix and post chrome with 2.5%
potassium dichromate for 2 days.  Process in paraffin and section as
usual.

1.  Bring sections to water with xylene and ethanol.
2.  If Zenker fixed, remove mercury pigment.
3.  Place in carbol ailine fuchsin for 5 minutes at 70C, or steaming on
a hot plate. 4. Rinse quickly with tap water. 5. Decolourise with strong
formalin (40%) a few drops at a time until no more colour is removed
(15-20 minutes) 6. Rinse with tap water. 7. Counterstain with saturated
aqueous picric acid for 1 minute. 8. Dehydrate with ethanol, clear with
xylene and mount with Clarite (or something similar).

Results:
    Encephalitozoa - blue black
    Toxoplasma chromatin - brown red
    Influenza bacilli - blue
    Nuclei - light red
    Cytoplasm - pink yellow
    Erythrocytes - bright yellow


Bryan Llewellyn



----- Original Message ----- 
From: "Kathleen Cormier" <cormier <@t> MIT.EDU>
To: <histonet <@t> pathology.swmed.edu>
Sent: Wednesday, March 24, 2004 11:35 AM
Subject: [Histonet] Goodpasture's carbol fuchsin stain


> Greetings!
>
> I have a researcher who is requesting a Goodpasture's carbol fuchsin 
> stain on rabbit kidneys to stain protoza. (encephalitozoon cuniculi or

> the old old name renal nosematosis). My research indicates giemsa and 
> gram would
be
> acceptable as well, but, I can find no reference for the Goodpasture's

> carbol fuchsin. I have exhausted histonet archives and my histo books
here.
> Can anyone point me in the direction of a procedure for the 
> Goodpasture's? Thanks!!!
>
>
> Kathy
>
> DCM MIT
>
>
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>



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