(Fwd) Re: [Histonet] EDTA pH 8.0 recipe

Barry R Rittman Barry.R.Rittman <@t> uth.tmc.edu
Wed Mar 24 09:19:21 CST 2004


Tammy and Greg
EDTA powder is only slightly soluble in water. I prefer to start with
disodium EDTA (that is much more soluble)and then adjust the pH to 8
with NaOH.
Can also use this in saline.
The 1.86 gms in 5 liters is a very weak solution for demineralization.
Normally we use 5% EDTA adjusted to pH 7.3.
If cost is a the determining factor then EDTA can be recovered from a
used demineralization solution - however very few do this because of the
time involved.
Barry


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Greg
Dobbin
Sent: Wednesday, March 24, 2004 5:01 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: (Fwd) Re: [Histonet] EDTA pH 8.0 recipe


Hi Tammy,
I maintain my own "personal" Histonet Archives, and I found the 
following post from 1999. Good luck.
Greg

Date sent:      	Mon, 27 Dec 1999 11:15:29 -0600
From:           	"Lott, Robert" <Robert.Lott <@t> bhsala.com>
Subject:        	EDTA-NaOH, pH 8.0 retrieval fluid
Forwarded to:   	DOBBIN <@t> acad1.cs.upei.ca
To:             	"'histonet <@t> pathology.swmed.edu'" 
<histonet <@t> pathology.swmed.edu>


Stock Solution:
	1.86 gm. EDTA (same as used for decalcification)
	5.0   liters DI water

To the above solution, add 2 to 2.5 ml. 2N NaOH.
Adjust solution to pH 8.0 with 1N NaOH or 1N HCl solution.
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

From:           	"Barnhart, Tammy" <Tbarnhart <@t> primecare.org>
To:             	"Histonet (E-mail)"
<histonet <@t> lists.utsouthwestern.edu>
Date sent:      	Wed, 24 Mar 2004 07:57:44 -0600
Subject:        	[Histonet] EDTA pH 8.0 recipe

> Does anyone have a recipe to make EDTA buffer at a pH of 8.0.  I would

> like to make our own in an effort to save some dollars.  I have 
> checked the archives and only have come up with one rather difficult 
> procedure. Thanks in advance....
> 
> Tammy Barnhart, BS, HTL(ASCP)
> Anatomic Pathology Supervisor
> St. Alexius Medical Center
> Bismarck, ND
> tbarnhart <@t> primecare.org
> 
> 
> 
> Confidentiality Notice:This e-mail message is for sole use of intended
> recipient(s) and may contain confidential and privileged information. 
> Any unauthorized review, use, disclosure, distribution, or copying is 
> prohibited. If you are not the intended recipient, please contact the 
> sender by replying to this e-mail and destroy/delete all copies of 
> this e-mail message. _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet




_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




More information about the Histonet mailing list